Coding

Part:BBa_K4320013

Designed by: Angela Gao   Group: iGEM22_MIT   (2022-10-11)


acsC-rbs-acsD

acsC and acsD are two of the four genes in the Acs cellulose synthesis operon in Gluconacetobacter xylinus. Their exact functions are yet to be fully characterized, but they are required for maximal bacterial cellulose synthesis. acsC may be involved in the "formation of a membrane complex for extrusion of the cellulose product" and acsD may have a "major role in the perfection of crystallization, involved either in the pore structure itself or in the organization of the pores within the linear array of terminal synthesizing complexes" (Uniprot).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1809
    Illegal BglII site found at 4354
    Illegal BamHI site found at 1878
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 750
    Illegal AgeI site found at 1306
    Illegal AgeI site found at 1885
    Illegal AgeI site found at 2095
    Illegal AgeI site found at 2947
    Illegal AgeI site found at 3265
    Illegal AgeI site found at 3610
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None