Plasmid_Backbone

Part:BBa_K4320008

Designed by: MIT iGEM 2022   Group: iGEM22_MIT   (2022-10-11)


pYTK_097

pYTK_097 is a level 1 or 2 pre-assembled S. cerevisiae LEU2 plasmid that we assembled using the Duber YTK MoClo Kit. It contains a GFP dropout sequence, is compatible with BsaI (level 1) and BsmBI (level 2) and has ampicillin resistance in E. coli and LEU selection for integration into S. cerevisiae. It was assembled from the parts ConLS, GFP dropout, ConR1, LEU2, 2micron, AmpR-ColE1 from the Duber YTK MoClo Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
    Illegal NotI site found at 4324
    Illegal NotI site found at 6182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
    Illegal AgeI site found at 2295
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1214
    Illegal BsaI.rc site found at 196
    Illegal SapI.rc site found at 380


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