Terminator

Part:BBa_K4316030

Designed by: Luca Donati   Group: iGEM22_Sorbonne_U_Paris   (2022-10-06)


Terminator.png

RbcS-2b terminator B6-C1


RbcS-2b terminator was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B6-C1.

This terminator could decrease protein expression 10-fold in Chlamydomonas Reinhardtii therefore improving cell viability [1].

This part’s sequence was retrieved from Crozet et al. [1].

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.


Secondary Structure

File:Mfold-K4316030-1.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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