Protein_Domain

Part:BBa_K4316026

Designed by: Luca Donati   Group: iGEM22_Sorbonne_U_Paris   (2022-10-06)


cCA secretion signal B2

The cCA secretion signal part was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B2.

The secretion signal of carbonic anhydrase (cCA) addresses proteins to the interior of the plasmic membrane. [2]

Sequence was inferred from UniProt accession number P20507.

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

[2] Fukuzawa H, Fujiwara S, Yamamoto Y, Dionisio-Sese ML, Miyachi S. cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4383-7. doi: 10.1073/pnas.87.11.4383. PMID: 2112252; PMCID: PMC54114.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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