Part:BBa_K4316021

GSAT linker B4

Similar to a part named BBa_K404301 made by iGEM Freiburg Bioware 2010.

GSAT Linker was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B4. Its 5’ scar is AGGT and its 3’ scar is TTCG.

This part is able to connect two protein domains and add additional space between them . The GSAT linker was created to fuse our protein domains and avoid rigidity and folding interaction between the two parts.

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 7
  Illegal NgoMIV site found at 67
  
• 1000
  COMPATIBLE WITH RFC[1000]