Part:BBa_K4316020

COP1 CDS B3

COP1 CDS was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B3 . Its 5’ scar is A (because start codon ATG is contained in this sequence) and its 3’ scar is AGGT.

The COP1 protein is an E3 ubiquitin ligase that induces the degradation of photoreceptors and other development related and light sensitive proteins by the proteasome.[2,3] It dimerizes with UVR8 under UV radiation.

Guanidine in position 390, 567, 915 and 930 were replaced with cytidine as to remove PstI sites, without changing the coding sequence, and make part compatible with RFC10 standard.

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

[2] Yamamoto YY, Matsui M, Ang LH, Deng XW. Role of a COP1 interactive protein in mediating light-regulated gene expression in arabidopsis. Plant Cell. 1998;10: 1083–1094. doi:10.1105/tpc.10.7.1083

[3] Kim JY, Song JT, Seo HS. COP1 regulates plant growth and development in response to light at the post-translational level. Raines C, editor. Journal of Experimental Botany. 2017;68: 4737–4748. doi:10.1093/jxb/erx312

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1218
  Illegal NgoMIV site found at 1924
  
• 1000
  COMPATIBLE WITH RFC[1000]