Part:BBa_K4316016

mVenus sequence for MoClo B2-B5 position

mVenus CDS was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B2-B5. It’s 5’ scar is CC (because start codon ATG is contained in this sequence) and it’s 3’ scar is GCTT.

mVenus is a yellow constitutively fluorescent protein first published in 2006, derived from Aequorea victoria. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity. [2]

Sequence retrieved from Crozet et al., 2018 [1]

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

[2] Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr. Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry. 2006 May 30;45(21):6570-80. doi: 10.1021/bi0516273. PMID: 16716067.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 766
  Illegal PstI site found at 235
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 766
  Illegal PstI site found at 235
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 766
  Illegal BglII site found at 10
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 766
  Illegal PstI site found at 235
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 766
  Illegal PstI site found at 235
  Illegal NgoMIV site found at 712
  
• 1000
  COMPATIBLE WITH RFC[1000]