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Part:BBa_K4316013

Designed by: Luca Donati   Group: iGEM22_Sorbonne_U_Paris   (2022-10-06)


SulatorReverse in MoClo C1 position

The reverse sulator sequence was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position C1. It’s 5’ scar is GGTA and it’s 3’ scar is CGCT.

This sequence was designed to serve as a control « buffer » when testing the Drosophila melanogaster reverse Gypsy insulator in Chlamydomonas reinhardtii (part number BBa K4316011). It serves no explicit function in this context. This part is the reverse complementary of part BBa K4316012.

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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