Part:BBa_K431010
Formaldehyde Dehydrogenase (FLD1) from ''Pichia pastoris''
Formaldehyde Dehydrogenase (FLD1) from Pichia pastoris
Formaldehyde is a convergent intermediate for utilization of both methanol as a single carbon source and methylamine as a single nitrogen source in Pichia pastoris. Formaldehyde Dehydrogenase is responsible for the oxidation of formaldehyde into formate and is required for cell survival when grown on either methanol or methylamine. For this reason FLD1 has been developed into an alternative marker gene for DNA transformations in fld1- P. pastoris strains.
It has also been shown that cultures with multiple copies of their expression cassette can be obtained by selecting for organisms with increased formaldehyde resistance. This is a similar concept to that used with increased antibiotic resistance but does not pose a biohazard risk. It also avoids multiple transformations, each of which requires a different marker gene.
Users can therefore use this part for selectivity of transformed cells and to obtain high copies of their desired expression cassette.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 238
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 238
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 238
Illegal BglII site found at 683 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 238
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 238
Illegal AgeI site found at 931 - 1000COMPATIBLE WITH RFC[1000]
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