Regulatory

Part:BBa_K4307041

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-10-13)


OR2-OR3

There are two operators in the genome of bacteriophage, called OR (the right operator) and OL (the left operator) respectively. OR plays an important role in the regulation of gene expression of phage. OR composed of three parts, called OR1, OR2 and OR3. The isolated OR2-OR3 can function as promoter and can be inhibited by Cro protein, a derepressor expressed by bacteriophage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


Figure 1: The construction results of OR2-OR3.


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Categories
Parameters
None