Regulatory

Part:BBa_K4303030

Designed by: Arya Ajit Gohad   Group: iGEM22_ICT-Mumbai   (2022-12-16)


5'UTR

This part is an RBS- UTR Hybrid with a lower expression than the RBS itself. This was tested in E. coli of two strains: DH5alpha and BL21(DE3), and the protein expressed was RFP. The comparative performance in the strain DH5alpha was 0.5468 compared to the control (RBS only), with a standard deviation of 0.0087. The comparative performance in the strain BL21(DE3) was 0.3400 compared to the control (RBS only), with a standard deviation of 0.0315.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
    Illegal PstI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
    Illegal PstI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
    Illegal PstI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None