Regulatory
Part:BBa_K4303030
Designed by: Arya Ajit Gohad Group: iGEM22_ICT-Mumbai (2022-12-16)
5'UTR
This part is an RBS- UTR Hybrid with a lower expression than the RBS itself. This was tested in E. coli of two strains: DH5alpha and BL21(DE3), and the protein expressed was RFP. The comparative performance in the strain DH5alpha was 0.5468 compared to the control (RBS only), with a standard deviation of 0.0087. The comparative performance in the strain BL21(DE3) was 0.3400 compared to the control (RBS only), with a standard deviation of 0.0315.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 60
Illegal PstI site found at 21 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 21
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 60
Illegal PstI site found at 21 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 60
Illegal PstI site found at 21 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |