Regulatory
Part:BBa_K4303011
Designed by: Arya Ajit Gohad Group: iGEM22_ICT-Mumbai (2022-09-29)
5' UTR
This is an RBS-UTR hybrid that gives lower expression than the RBS alone. This was tested in E. coli, and the protein expressed was RFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 21
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 21
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 21
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 21
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |