Regulatory

Part:BBa_K4303011

Designed by: Arya Ajit Gohad   Group: iGEM22_ICT-Mumbai   (2022-09-29)


5' UTR

This is an RBS-UTR hybrid that gives lower expression than the RBS alone. This was tested in E. coli, and the protein expressed was RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 21
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None