Composite

Part:BBa_K4297085

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


pET-Alloferon-1-eGFP-SD-AmpR

The composite part contains Lambda cI , cytidine deaminase, Uracil DNA glycosylase inhibitor and LVA degradation tag. CDA catalyzes the deamination of cytosine in the top (non-complementary) strand located within 15-20 bases upstream of PAM, resulting in a C-T mutation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 889
    Illegal BsaI site found at 1615
    Illegal BsaI.rc site found at 1766


[edit]
Categories
Parameters
None