Coding

Part:BBa_K4297082

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


lacZ-sgRNA

The related genes of the sgRNA plasmid can be targeted to the lacZ.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 139
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 139
    Illegal BglII site found at 169
    Illegal XhoI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None