Coding

Part:BBa_K4297070

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


Ecb

The gene encoding a membrane protein (F-ATPase subunit b) whose expression in E. coli BL21(DE3) interferes with normal host growth resulting in low yield.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 488
    Illegal SapI.rc site found at 363


[edit]
Categories
Parameters
None