Coding

Part:BBa_K4297023

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


Leu

Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of beta-ethylmalate into alpha-ketovalerate


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
    Illegal XhoI site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 327
    Illegal BsaI site found at 610
    Illegal BsaI.rc site found at 525
    Illegal BsaI.rc site found at 1235
    Illegal SapI site found at 1078


[edit]
Categories
Parameters
None