Composite
Part:BBa_K4294808
Designed by: Aristotelis Anastopoulos Group: iGEM22_Athens (2022-10-12)
TU constitutive LuxR for OL
Usage and Biology
It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.
Design
We used this transcriptional unit in our Open loop construct (OL) (BBa_K4294801)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |