Composite

Part:BBa_K4294807

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-10-09)


TU pLuxr PhlF

Design

We used this transcriptional unit in our Positive Feedback+ Repressor (BBa_K4294804) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs. PhlF was chosen for our constructs since it is an effective repressor with a low association constant K, indicating its ability to form functional dimers in lower concentrations (according to the Ecoli Marionette strains characterization [1])

Reference

[1] Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26. PMID: 30478458.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None