Composite

Part:BBa_K4294790

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-09-30)


TetX TU

This is the transcriptional unit encoding for the TetX enzyme that belongs to the tetracycline destructase family, a group of enzymes that degrade tetracycline and its derivatives, such as aTc[1].

Circuit Design

We utilized pTKEI-Dest as the acceptor vector of the construct we built to tackle the cross-talk issue among discrete senders’ subpopulations. It includes a kanamycin (Kan) resistance gene and measures approximately 4.5kbp in length. It is a high-copy plasmid embodying the pBR322 origin of replication. This TU has the TetX gene controlled by the inducible by IPTG pTac promoter and the BBa_B0034 RBS.

Measurment

There has been no measurement for this part.

References

[1]Markley JL, Wencewicz TA. Tetracycline-Inactivating Enzymes. Front Microbiol. 2018 May 30;9:1058. doi: 10.3389/fmicb.2018.01058. PMID: 29899733; PMCID: PMC5988894.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None