Part:BBa_K4294790
TetX TU
This is the transcriptional unit encoding for the TetX enzyme that belongs to the tetracycline destructase family, a group of enzymes that degrade tetracycline and its derivatives, such as aTc[1].
Circuit Design
We utilized pTKEI-Dest as the acceptor vector of the construct we built to tackle the cross-talk issue among discrete senders’ subpopulations. It includes a kanamycin (Kan) resistance gene and measures approximately 4.5kbp in length. It is a high-copy plasmid embodying the pBR322 origin of replication. This TU has the TetX gene controlled by the inducible by IPTG pTac promoter and the BBa_B0034 RBS.
Measurment
There has been no measurement for this part.
References
[1]Markley JL, Wencewicz TA. Tetracycline-Inactivating Enzymes. Front Microbiol. 2018 May 30;9:1058. doi: 10.3389/fmicb.2018.01058. PMID: 29899733; PMCID: PMC5988894.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |