Part:BBa_K4294401

LuxR Activator/Repressor Codon Optimised for E Coli

This is the coding sequence for the LuxR repressor, codon optimized for E.coli. It controls the pLux promoter.

Biological Role

It can be used in the QS system of LuxI/LuxR. LuxI synthase catalyzes the production of 3-oxohexanoyl-homoserine lactone (OC6 for short), which belongs to a family of QS molecules called N-Acetyl-Homoserin-Lactones (AHLs). An activated bacterial population can express LuxI and the produced OC6 molecules diffuse outside of this bacterial population to activate another one. Inside the second population, OC6 molecules bind to LuxR, a transcriptional regulator that forms its functional dimer after binding with OC6, which activates the Plux promoter and, therefore, the downstream gene.

We used it for our receivers' constructs like OL (BBa_K4294801), PF (BBa_K4294802), PFR (BBa_K4294804), PFc (BBa_K4294803), PFc+R (BBa_K4294805).

References

[1] Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of bacteriology, 176(2), 269–275. https://doi.org/10.1128/jb.176.2.269-275.1994

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]