Regulatory
Part:BBa_K4294301
Designed by: Aristotelis Anastopoulos Group: iGEM22_Athens (2022-09-29)
LuxR-Phlf hybrid promoter
Design
This new promoter was used in our design attempts to create a genetic circuit with a steeper response curve. It contains a LuxR binding domain (lux box) upstream the -35 region and a Phlf operator in the core sequence that also overlaps with the -10 region. It is therefore positively regulated by LuxR and negatively by Phlf.[1][2]
Plux-phlO hybrid promoter.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |