Coding

Part:BBa_K4294210

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-09-30)


LuxI codon optimised for E Coli (+6His Tag)

This gene encodes for the Acyl-Homoserine Lactone Synthase the enzyme responsible for OC6 quorum sensing molecule production. It is codon optimized for E.coli.

Measurment

For the measurement of the expression of the luxI gene, we incorporated in our design a 6*His tag that can be used either or immunological techniques (western blotting, ELISA) for the detection with anti-His antibody or for purification with affinity chromatography.

References

Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of bacteriology, 176(2), 269–275. https://doi.org/10.1128/jb.176.2.269-275.1994

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 586
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None