Coding
Part:BBa_K4294210
Designed by: Aristotelis Anastopoulos Group: iGEM22_Athens (2022-09-30)
LuxI codon optimised for E Coli (+6His Tag)
This gene encodes for the Acyl-Homoserine Lactone Synthase the enzyme responsible for OC6 quorum sensing molecule production. It is codon optimized for E.coli.
Measurment
For the measurement of the expression of the luxI gene, we incorporated in our design a 6*His tag that can be used either or immunological techniques (western blotting, ELISA) for the detection with anti-His antibody or for purification with affinity chromatography.
References
Fuqua, W. C., Winans, S. C., & Greenberg, E. P. (1994). Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. Journal of bacteriology, 176(2), 269–275. https://doi.org/10.1128/jb.176.2.269-275.1994
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 586
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |