Coding

Part:BBa_K4294204:Design

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-09-30)


36nt PMK - sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 23
    Illegal XhoI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References

Duan Y, Zhang X, Zhai W, Zhang J, Zhang X, Xu G, Li H, Deng Z, Shi J, Xu Z. Deciphering the Rules of Ribosome Binding Site Differentiation in Context Dependence. ACS Synth Biol. 2022 Aug 19;11(8):2726-2740. doi: 10.1021/acssynbio.2c00139. Epub 2022 Jul 25. PMID: 35877551.