Primer

Part:BBa_K4294077

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-10-12)


v59 RBS oligo Forward primer

This primer was used for primer annealing and extension in order to build the V59 RBS (BBa_K4294559).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 46


[edit]
Categories
Parameters
None