Primer

Part:BBa_K4294021

Designed by: Aristotelis Anastopoulos   Group: iGEM22_Athens   (2022-10-12)


Biobrick Prefix F

Forward primer used for PCR amplification of DNA parts with flanking Biobrick Prefix

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None