Translational_Unit

Part:BBa_K4291017

Designed by: Kong Yangyang   Group: iGEM22_Canton_HS   (2022-08-14)


J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro- SLC7A5- rrnB T1 ter- T7Te ter

J23119 pro-CueR- rrnB T1 ter- T7Te ter- CopA pro- SLC7A5- rrnB T1 ter- T7Te ter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1221
    Illegal PstI site found at 679
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 573
    Illegal PstI site found at 679
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1221
    Illegal PstI site found at 679
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1221
    Illegal PstI site found at 679
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1020
    Illegal SapI site found at 1587


[edit]
Categories
Parameters
None