Part:BBa_K4286527

cbh1 leader sequence

cbh1 promoter, which was first discovered in Trichoderma reesei, is one of the strongest inducible promoters in the fungal kingdom. We selected a known 1360bp cbh1 promoter sequence from the cbh1 gene of Trichoderma koningii, retaining the inducible regulatory site of polysaccharide and glucose. We also retained the 17bp leader sequence between the cbh1 promoter and cbh1 gene.

[1]Wey TT, Hseu TH, Huang L. Molecular cloning and sequence analysis of the cellobiohydrolase I gene from Trichoderma koningii G-39. Curr Microbiol. 1994 Jan;28(1):31-9. doi: 10.1007/BF01575983.

[2]Karhunen T, Mäntylä A, Nevalainen KM, Suominen PL. High frequency one-step gene replacement in Trichoderma reesei. I. Endoglucanase I overproduction. Mol Gen Genet. 1993 Dec;241(5-6):515-22. doi: 10.1007/BF00279893.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]