Part:BBa_K4286408

cbh1 promoter

cbh1 promoter, which was first discovered in Trichoderma reesei, is one of the strongest inducible promoters in the fungal kingdom. It is often used to construct expression vectors. The cbh1 promoter sequence contains cellulose and sophorose inducible binding sites and glucose feedback inhibitory binding sites. These sites are in different positions, which provide multiple possibilities for us to regulate the expression of genes downstream of the promoter. We selected a known 1360bp cbh1 promoter sequence from the cbh1 gene of Trichoderma koningii, retaining the inducible regulatory site of polysaccharide and glucose.

[1]Kluge J, Terfehr D, Kück U. Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi. Appl Microbiol Biotechnol. 2018 Aug;102(15):6357-6372. doi: 10.1007/s00253-018-9115-1.

[2]Wey TT, Hseu TH, Huang L. Molecular cloning and sequence analysis of the cellobiohydrolase I gene from Trichoderma koningii G-39. Curr Microbiol. 1994 Jan;28(1):31-9. doi: 10.1007/BF01575983.

[3]Madhavan A, Sukumaran RK. Promoter and signal sequence from filamentous fungus can drive recombinant protein production in the yeast Kluyveromyces lactis. Bioresour Technol. 2014 Aug;165:302-8. doi: 10.1016/j.biortech.2014.03.002. Epub 2014 Mar 12.

[4]Henrique-Silva F, el-Gogary S, Carle-Urioste JC, Matheucci E Jr, Crivellaro O, el-Dorry H. Two regulatory regions controlling basal and cellulose-induced expression of the gene encoding cellobiohydrolase I of Trichoderma reesei are adjacent to its TATA box. Biochem Biophys Res Commun. 1996 Nov 12;228(2):229-37. doi: 10.1006/bbrc.1996.1646. PMID: 8920899.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1006
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]