Plasmid

Part:BBa_K4284035

Designed by: Zhang Xinchi   Group: iGEM22_PINGHE   (2022-08-11)


pET28a-PT7_C4

pET28a-PT7_C4

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 977
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 207
    Illegal NotI site found at 180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 207
    Illegal BglII site found at 1028
    Illegal BamHI site found at 213
    Illegal XhoI site found at 173
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 977
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 977
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 2648
    Illegal NgoMIV site found at 2808
    Illegal NgoMIV site found at 5855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287
    Illegal SapI site found at 3728


[edit]
Categories
Parameters
None