Plasmid

Part:BBa_K4284032

Designed by: Zhang Xinchi   Group: iGEM22_PINGHE   (2022-08-11)


pET28a-EGFP

pET28a-EGFP

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1725
    Illegal XbaI site found at 1778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1725
    Illegal NotI site found at 1698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1725
    Illegal BglII site found at 1844
    Illegal BamHI site found at 1731
    Illegal XhoI site found at 1691
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1725
    Illegal XbaI site found at 1778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1725
    Illegal XbaI site found at 1778
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 3464
    Illegal NgoMIV site found at 3624
    Illegal NgoMIV site found at 5855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69
    Illegal SapI site found at 4544


[edit]
Categories
Parameters
None