Plasmid_Backbone

Part:BBa_K4284021

Designed by: Zhang Xinchi   Group: iGEM22_PINGHE   (2022-08-11)


pACYC-acs-backbone

pACYC-acs-backbone

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 83
    Illegal XbaI site found at 2564
    Illegal PstI site found at 102
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 83
    Illegal NheI site found at 1723
    Illegal PstI site found at 102
    Illegal NotI site found at 120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 83
    Illegal BglII site found at 276
    Illegal BamHI site found at 77
    Illegal XhoI site found at 325
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 83
    Illegal XbaI site found at 2564
    Illegal PstI site found at 102
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 83
    Illegal XbaI site found at 2564
    Illegal PstI site found at 102
    Illegal NgoMIV site found at 295
    Illegal AgeI site found at 537
    Illegal AgeI site found at 1809
    Illegal AgeI site found at 2132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None