Part:BBa_K4284017

pET28a-EGFP-backbone

pET28a-EGFP-backbone

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 962
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal NotI site found at 165
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal BglII site found at 1013
  Illegal BamHI site found at 198
  Illegal XhoI site found at 158
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 962
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 192
  Illegal XbaI site found at 962
  Illegal NgoMIV site found at 1045
  Illegal NgoMIV site found at 2633
  Illegal NgoMIV site found at 2793
  Illegal NgoMIV site found at 5840
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 272
  Illegal SapI site found at 3713