Part:BBa_K4281002

pKC1139-backbone

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4402
Illegal PstI site found at 1244
Illegal PstI site found at 2164
Illegal PstI site found at 7735
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4402
Illegal NheI site found at 6682
Illegal NheI site found at 7719
Illegal PstI site found at 1244
Illegal PstI site found at 2164
Illegal PstI site found at 7735
Illegal NotI site found at 1254
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4402
Illegal BglII site found at 3952
Illegal XhoI site found at 6186
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4402
Illegal PstI site found at 1244
Illegal PstI site found at 2164
Illegal PstI site found at 7735
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4402
Illegal PstI site found at 1244
Illegal PstI site found at 2164
Illegal PstI site found at 7735
Illegal NgoMIV site found at 292
Illegal NgoMIV site found at 342
Illegal NgoMIV site found at 490
Illegal NgoMIV site found at 888
Illegal NgoMIV site found at 2826
Illegal NgoMIV site found at 3262
Illegal NgoMIV site found at 3272
Illegal AgeI site found at 168
Illegal AgeI site found at 1162
Illegal AgeI site found at 7100
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 423
Illegal BsaI site found at 2720
Illegal BsaI.rc site found at 1862
Illegal BsaI.rc site found at 2950
Illegal SapI site found at 4634
Illegal SapI.rc site found at 3369
Illegal SapI.rc site found at 3738
Illegal SapI.rc site found at 6242
Illegal SapI.rc site found at 6738