Part:BBa_K4274006

PvanP*

The vanP* promoter (PvanP*) is situated at the upstream of pJOE8999 plamid and is used to transcribe sgRNA in CRISPR-Cas9 system (Josef et al., 2016).

Usage and Biology

The semi synthetic promoter PvanP* is used in our experiment to transcribe the gene coded for sgRNA in our CRISPR-Cas9 system. For the constitutive expression of sgRNA (patB gene and sfp gene) in pJOE8999, the semi-synthetic promoter PvanP* was used (Morabbi et al., 2015).

In the future, other teams may be able to use this part to transcribe sgRNA in pJOE8999, allowing for the gene-editing of B. subtilis genome.

Source

Corynebacterium glutamicum

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Morabbi heravi, K., Lange, J., Watzlawick, H., Kalinowski, J., & Altenbuchner, J. Transcriptional regulation of the vanillate utilization genes (vanABK operon) of corynebacterium glutamicum by VanR, a PadR-like repressor. Journal of Bacteriology, 197(5), 959-972(2015). https://doi.org/10.1128/JB.02431-14
[2] Josef Altenbuchner. Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system. Applied and Environmental Microbiology, 82(17), 5421-5427(2016). https://doi.org/10.1128/AEM.01453-16