Coding

Part:BBa_K4270010

Designed by: Haoting Xie   Group: iGEM22_GDSYZX   (2022-07-26)


PTS-linker-FPS

PTS-linker-FPS:a gene fusion of PTS and FPS.The stop codon of FPS was removed and replaced by a short peptide (Gly-Ser-Gly) to introduce a linker between the FPS and PTS ORFs[1,2]. Reference:[1] WU S, SCHALK M, CLARK A, et al. Redirection of cytosolic or plastidic isoprenoid precursors elevates terpene production in plants [J]. Nat Biotechnol, 2006, 24(11): 1441-7. [2] BRODELIUS M, LUNDGREN A, MERCKE P, et al. Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum [J]. Eur J Biochem, 2002, 269(14): 3570-7.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None