Part:BBa_K4270007

erg20 K197P (a mutant of ERG20 gene: K197P substitution)

erg20 K197P:a mutant of ERG20 gene; K197P substitution. Yeast strain K197P showslower sterol content compared with the original strain K197K[1].FPP is theprecursor of yeast steroland patchoulolbiosynthetic pathway.[2, 3] Reference:[1] FISCHER M J, MEYER S, CLAUDEL P, et al. Metabolic engineering of monoterpene synthesis in yeast [J]. Biotechnol Bioeng, 2011, 108(8): 1883-92. [2] SZKOPINSKA A, D. P. Farnesyl diphosphate synthase; regulation of product specificity [J]. Acta Biochim Pol, 2005, 52(45-55. [3] YU Z X, WANG L J, ZHAO B, et al. Progressive regulation of sesquiterpene biosynthesis in Arabidopsis and Patchouli (Pogostemon cablin) by the miR156-targeted SPL transcription factors [J]. Mol Plant, 2015, 8(1): 98-110.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]