Composite

Part:BBa_K426022

Designed by: Daniela Mehech   Group: iGEM10_Berkeley   (2010-10-27)

{J23100.1}{rbs_LifeAct_GDPPVAT>}.{GFP!}{rbs_RFP!}b1006

Lifeact is a marker for actin. This part associates Lifeact with GFP to make the actin filaments in an eukaryotic cell fluoresce green. The part also includes RFP, although it is expressed on its own.

Assay: This part is functional since it causes E. coli to express both GFP and RFP. It also causes an elongated phenotype, which we expect is due to the lifeact binding to the actin equivalent in E. coli and disrupting cell division.

Here's a link to more information on Lifeact: http://www.nature.com/nmeth/journal/v5/n7/full/nmeth.1220.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 15
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1449
    Illegal AgeI site found at 1561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


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Categories
Parameters
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