Composite

Part:BBa_K426018

Designed by: Daniela Mehech   Group: iGEM10_Berkeley   (2010-10-26)


Pbad{rbs_pelB>},tn5,{NIS!}b1006Tn5 5

complete tn5, without any zinc finger, and with self-lysis

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2261
    Illegal NheI site found at 4105
    Illegal NheI site found at 5746
    Illegal NheI site found at 5769
    Illegal PstI site found at 1926
    Illegal NotI site found at 1839
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1926
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 4043
    Illegal NgoMIV site found at 6131
    Illegal AgeI site found at 1028
    Illegal AgeI site found at 2249
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2393
    Illegal BsaI.rc site found at 2214
    Illegal BsaI.rc site found at 2913
    Illegal SapI site found at 1010
    Illegal SapI site found at 1350
    Illegal SapI.rc site found at 1662


[edit]
Categories
Parameters
None