Composite

Part:BBa_K426016

Designed by: Daniela Mehech   Group: iGEM10_Berkeley   (2010-10-26)


Pbad{rbs_pelB>},zf+,piggyBac!b1006piggieBac_5

Complete piggieBac with zf+ and Self-Lysis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5318
    Illegal NheI site found at 6959
    Illegal NheI site found at 6982
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 5256
    Illegal NgoMIV site found at 7344
    Illegal AgeI site found at 1028
    Illegal AgeI site found at 2226
    Illegal AgeI site found at 2738
    Illegal AgeI site found at 3024
    Illegal AgeI site found at 3386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3920
    Illegal SapI site found at 1010


[edit]
Categories
Parameters
None