Coding

Part:BBa_K426011

Designed by: Daniela Mehech   Group: iGEM10_Berkeley   (2010-10-26)


SB100x! (Sleeping Beauty)

This part is derived from PMID 19412179. It is a hyperactive mutant of the originally described sleeping beauty transposase. This part encodes a DNA modifying enzyme. This part is associated with sleeping beauty transposon devices. Sleeping Beauty transposase is a popular enzyme that generates protein-DNA complexes called transposomes from DNAs flanked by "terminal repeats". These terminal repeats, or TR's, are specific cis elements. The transposomes are highly reactive and insert the DNA contained within them randomly into other DNAs (such as a genome).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 282
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None