Plasmid

Part:BBa_K4258060

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-10-09)


J23102+BCD2+CDS#20+B0015+DVK

The Golden Gate product of the following parts: J23102 Promoter (BBa_J23102) from the MoClo CIDAR Kit + BCD2 RBS (BBa_K1114107) from the MoClo CIDAR Kit + Synthesized CDS #20 (BBa_K4258020) + B0015 Terminator (BBa_B0015) from the CIDAR MoClo Kit + DVK_AE Backbone from the CIDAR MoClo Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 143
    Illegal BsaI.rc site found at 873


[edit]
Categories
Parameters
None