Plasmid

Part:BBa_K4258050

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-10-09)


AraC+CDS#20+B0015+DVK

The Golden Gate product of the following parts: AraC Inducible Promoter (BBa_K4258006) + Synthesized CDS #20 (BBa_K4258020) + B0015 Terminator (BBa_B0015) from the CIDAR MoClo Kit + DVK_AE Backbone from the CIDAR MoClo Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1053
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1333
    Illegal BsaI.rc site found at 1312
    Illegal BsaI.rc site found at 2063
    Illegal SapI site found at 1035


[edit]
Categories
Parameters
None