Coding

Part:BBa_K4258016

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


His-mScarlet-Linker-CBDfimi

Bsa1 sites for Golden Gate cloning as a CD module and Bpi1 sites for cloning into pOGG006 to immortalize the part as a plasmid. 6x Histidine tag + mScarlet from Addgene Plasmid #121003 + 10x GS Linker + Cellulose Binding Domain from Cellulomonas fimi (BBa_K863101). A coding sequence that will express m-Scarlet-I (bright pink) with a cellulose binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1103


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Parameters
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