Plasmid_Backbone

Part:BBa_K4251023

Designed by: Shi Lifang   Group: iGEM22_SubCat_Shanghai   (2022-09-21)


GV248

GV248

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 4265
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 2756
    Illegal BglII site found at 3595
    Illegal BglII site found at 5369
    Illegal BglII site found at 5639
    Illegal BglII site found at 8329
    Illegal BglII site found at 8703
    Illegal BamHI site found at 5347
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 185
    Illegal NgoMIV site found at 246
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 8083
    Illegal NgoMIV site found at 9282
    Illegal AgeI site found at 6812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 805
    Illegal SapI.rc site found at 4229


[edit]
Categories
Parameters
None