Part:BBa_K4245133

Lettuce Aptamer

This part is the full sequence of the fluorescent DNA aptamer Lettuce, developed by the Weill-Cornell Medical College. Lettuce is a 99 nucleotide long DNA aptamer that induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).

This part can be split into a left (BBa_K4245134) and right (BBa_K4245135) component so that fluorescence is induced when the two components come together in the presence of a target sequence (VarnBuhler et al., 2022). The fluorescence-inducing main sequence of Lettuce is kept the same while flanking sequences are added to the left and right components depending on the target sequence (VarnBuhler et al., 2022).

References

VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]