RNA
miR-150-5p

Part:BBa_K4245010

Designed by: Akshaya Poonepalle, Michelle Jing, Shivaek Venkateswaran, Daeun Lee, Sahana Ram Narayanan, Richard Jiang, Sishnukeshav Balamurali, Christina Yi, Varnica Basavaraj, Manaswi Gorle, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-09-22)

hsa-miR-150-5p

This part is the sequence for hsa-miR-150-5p, an miRNA isolated from Homo sapiens. This miRNA acts as a biomarker for coronary artery disease and is therefore potentially useful for the early detection of this disease. hsa-miR-150-5p is related to the inflammation process and is involved in the pathogenesis of coronary artery disease. Therefore, hsa-miR-150-5p is a means by which cardiologists can monitor the progression of the disease in patients (Zhang et al., 2015). However, hsa-miR-150-5p seems to be dysregulated, increased and decreased in concentration. Nevertheless, more research, with larger sample sets is needed to see if this biomarker is viable for CAD diagnosis (Kaur et al., 2020).

The Lambert_GA 2022 team developed a set of padlock probes to use the rolling circle amplification approaches for several miRNAs related to CAD. This miRNA is used as the basis for BBa_K4245104 and BBa_K4245111, the 3' arm for hsa-miR-150-5p and 5' arm for hsa-miR-150-5p, and as the target sequence for BBa_K4245206 the hsa-miR-150-5p RCA Padlock Probe.

When using rolling circle amplification (RCA), the miRNA binds to the padlock. A rolling circle product (RCP) is produced from BBa_K4245131 (Middle Sequence), which is then detected by the linear probes BBa_K4245130 (Fluorophore) and BBa_K4245132 (Quencher). When these parts bind to the RCP, the fluorescence decreases. Therefore, lower fluorescence is indicative of greater miRNA concentrations.

References:

Kaur, A., Mackin, S. T., Schlosser, K., Wong, F. L., Elharram, M., Delles, C., Stewart, D. J., Dayan, N., Landry, T., & Pilote, L. (2020). Systematic review of microRNA biomarkers in acute coronary syndrome and stable coronary artery disease. Cardiovascular research, 116(6), 1113–1124. https://doi.org/10.1093/cvr/cvz302
Zhang, R., Lan, C., Pei, H., Duan, G., Huang, L., & Li, L. (2015). Expression of circulating miR-486 and miR-150 in patients with acute myocardial infarction. BMC cardiovascular disorders, 15, 51. https://doi.org/10.1186/s12872-015-0042-0

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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