Composite

Part:BBa_K4244066

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-12)


Pm promoter:FimH:RPM

Pm promoter from Pseudomonas putida. Adhesion domain FimH of Type 1 fimbriae of Escherichia coli K-12. Which is fused with RPM at C terminus. RPM is a 9 amino acids sequence (CPIEDRPMC), that binds specifically to integrin α5β1 in human invasive colon cancer cells [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 131
    Illegal NotI site found at 121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 131
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Lee YM, Lee D, Kim J, Park H, Kim WJ. RPM peptide conjugated bioreducible polyethylenimine targeting invasive colon cancer. J Control Release [Internet]. 2015;205:172–80. Available from: http://dx.doi.org/10.1016/j.jconrel.2015.01.020

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