Regulatory
Part:BBa_K4244057
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-09)
rbcL: Ribulose bisphosphate carboxylase large subunit
The sequences include the promoter and the 5-UTR. The sequences were amplified by PCR from the total DNA of the wild-type Phaeodactylum tricornutum CCAP 1055/1. Chloroplast promoters are eubacterial in origin and thus compatible with gene expression in bacteria [1,2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Gatenby AA, Rothstein SJ, Bradley D. Using bacteria to analyze sequences involved in chloroplast gene expression. Photosynth Res. 1988;19(1–2):7–22. 2. Xie WH, Zhu CC, Zhang NS, Li DW, Yang WD, Liu JS, et al. Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum. Mar Biotechnol. 2014;16(5):538–46.
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Categories
Parameters
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