Reporter
Part:BBa_K4244055
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-08)
Three-step inducible system with BFP
This plasmid contains one component out of three of the three-step inducible system. Here, the behaviour of the system with different lengths of the t5 spacer can be tested by measuring the BFP fluorescence. This construct consists of BFP coding sequence with a dSpyCas9 target site in between the promoters and coding sequence of this protein. Additionally, an introduction template for sgRNA spacers was introduced. This template contains two type IIS restriction sides, this way different length t5 spacers can be easily introduced and tested.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 151
Illegal NheI site found at 7
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 151
Illegal BglII site found at 181
Illegal BamHI site found at 849 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 151
Illegal XbaI site found at 157
Illegal SpeI site found at 30
Illegal PstI site found at 169 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |