Reporter

Part:BBa_K4244053

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


Lactate inducible sfGFP

This is a composite part that is responsive to lactate and outputs super folder green fluorescent protein (sfGFP). It contains the transcription factor LldR which can bind to operator sites up- and downstream of the lactate inducible promoter A Lactate Promoter Operating in Glucose and Anoxia (ALPaGA). If lactate is absent LldR binds to the operator sites meaning the promoter is repressed and sfGFP is not transcribed. However if lactate is present, LldR changes conformation which changes it into an activator, meaning sfGFP is expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 56
    Illegal SpeI site found at 36
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 56
    Illegal SpeI site found at 36
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 56
    Illegal SpeI site found at 36
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1452


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Categories
Parameters
None